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| Registros recuperados : 18 | |
| 11. |  | CASTRO, G. M. de; VILLANOVA, D. F. Q.; LIMA, J. C. F.; OTENIO, M. H.; CARNEIRO, J. da C. Aclimatação de Inóculo para determinação de PME. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 17., 2016, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2016. 1 CD-ROM. (Embrapa Gado de Leite. Documentos, 186).| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | CASTRO, G. M. de; BOITEUX, L. S.; FONSECA, M. E. N.; LOBO, F.; RECH, E.; SILVA, F. R. da. Serendipitomics: a new virus found on an RNA-seq library. In: PLANT & ANIMAL GENOME CONFERENCE, 22., 2014, San Diego, CA. [Abstracts]. San Diego: [s.n.], 2014. Não paginado. P018.| Biblioteca(s): Embrapa Hortaliças. |
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| 13. | | GIACHETTO, P. F.; NHANI JUNIOR, A.; CASTRO, G. M. de; RODRIGUES, V. da S.; GARCIA, M. V.; BARROS, J. C.; ANDREOTTI, R. Differential gene expression in the cattle tick Rhipicephalus microplus fed on resistant and susceptible cattle. In: PLANT AND ANIMAL GENOME CONFERENCE, 27., 2019, San Diego. Abstracts... [S.l.: s.n.], 2019. Na publicação: Jacqueline Cavalcanti Barros, Renato Andreotti. PAG 2019. PE0246.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 14. | | CASTRO, G. M. de; BOITEUX, L. S.; FONSECA, M. E. de N. F.; LOBO, F.; RECH, E.; SILVA, F. R. da. Serendipitomics: a new virus found on an RNA-seq library. In: PLANT & ANIMAL GENOME CONFERENCE, 22., 2014, San Diego, CA. [Abstracts]. San Diego: [s.n.], 2014. Não paginado. P018.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | CASTRO, G. M. de; BOITEUX, L. S.; FONSECA, M. E. de N. F.; LOBO, F.; RECH, E.; SILVA, F. R. da. Serendipitomics: a new virus found on an RNA-seq library. In: PLANT & ANIMAL GENOME CONFERENCE, 22., 2014, San Diego, CA. [Abstracts...]. San Diego: [s.n.], 2014. Não paginado. P018.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 16. | | BELESINI, A. A.; CARVALHO, F. M. S.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. S.; CARLIN, S. D.; CAZETTA, J. O.; PINHEIRO, D. G.; FERRO, M. I. T. De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress. Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 2, p. 1-20, 2017. Article gmr16028845.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 17. | | BELESINI, A. A.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. da S.; CARVALHO, F. M. de S.; CARLIN, S. R.; CAZETTA, J. O.; FERRO, M. I. T.; PINHEIRO, D. G. de novo assembly and transcriptome analysis of sugarcane leaves from contrasting varieties submited to prolonged water stress. In: PLANT & ANIMAL GENOME CONFERENCE, 24., 2016, San Diego, CA. [Abstracts...]. San Diego: [s.n.], 2016. Não paginado. PAG 2016. Pôster P0792.| Biblioteca(s): Embrapa Agricultura Digital. |
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| 18. | | TELLES, B. R.; CARVALHO, F. M. de S.; VANTINI, J. da S.; BELESINI, A. A.; CASTRO, G. M. de; GIACHETTO, P. F.; CARLIN, S. D.; SILVA, T. R. da; PINHEIRO, D. G.; CAZETTA, J. O.; FERRO, M. I. T. Prolonged water deficit reveals new profile of sugarcane gene expression and metabolic pathway related to tolerance. Sugar Tech, v. 21, n. 3, p. 451-461, May-June 2019.| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 18 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
18/12/2017 |
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Data da última atualização: |
18/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BELESINI, A. A.; CARVALHO, F. M. S.; TELLES, B. R.; CASTRO, G. M. de; GIACHETTO, P. F.; VANTINI, J. S.; CARLIN, S. D.; CAZETTA, J. O.; PINHEIRO, D. G.; FERRO, M. I. T. |
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Afiliação: |
FCAV/Unesp; FCAV/Unesp; FCAV/Unesp; POLIANA FERNANDA GIACHETTO, CNPTIA; FCAV/Unesp; Centro Avançado da Pesquisa Tecnológica do Agronegócio de Cana, Ribeirão Preto, SP; FCAV/Unesp; FCAV/Unesp; FCAV/Unesp. |
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Título: |
De novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 16, n. 2, p. 1-20, 2017. |
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DOI: |
http://dx.doi.org/10.4238/gmr16028845 |
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Idioma: |
Inglês |
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Notas: |
Article gmr16028845. |
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Conteúdo: |
ABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 days. Both cultivars perceived the lack of water, but the tolerant cultivar responded more slowly than the sensitive cultivar. The latter requires rapid activation of different water-deficit stress response mechanisms for its survival. This rapid activation of metabolic pathways in response to water stress does not appear to be the key mechanism of drought tolerance in sugarcane. There is still much to clarify on the molecular and physiological pattern of plants in response to drought. MenosABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 da... Mostrar Tudo |
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Palavras-Chave: |
Expressão genética. |
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Thesagro: |
Cana de açúcar; Deficiência hídrica. |
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Thesaurus NAL: |
Drought; Gene expression; Saccharum; Sugarcane; Water stress. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/169187/1/AP-Denovo-Belesini-gmr.pdf
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Marc: |
LEADER 03036naa a2200349 a 4500
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008 2017 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr16028845$2DOI
100 1 $aBELESINI, A. A.
245 $aDe novo transcriptome assembly of sugarcane leaves submitted to prolonged water-deficit stress.$h[electronic resource]
260 $c2017
500 $aArticle gmr16028845.
520 $aABSTRACT. Sugarcane production is strongly influenced by drought, which is a limiting factor for agricultural productivity in the world. In this study, the gene expression profiles obtained by de novo assembly of the leaf transcriptome of two sugarcane cultivars that differ in their physiological response to water deficit were evaluated by the RNA-Seq method: drought-tolerant cultivar (SP81-3250) and drought-sensitive cultivar (RB855453). For this purpose, plants were grown in a greenhouse for 60 days and were then submitted to three treatments: control (-0.01 to -0.015 MPa), moderate water deficit (-0.05 to -0.055 MPa), and severe water deficit (-0.075 to -0.08 MPa). The plants were evaluated 30, 60, and 90 days after the beginning of treatment. Sequencing on an Illumina platform (RNA-Seq) generated more than one billion sequences, resulting in 177,509 and 185,153 transcripts for the tolerant and sensitive cultivar, respectively. These transcripts were aligned with sequences from Saccharum spp, Sorghum bicolor, Miscanthus giganteus, and Arabidopsis thaliana available in public databases. The differentially expressed genes detected during the prolonged period of water deficit permit to increase our understanding of the molecular patterns involved in the physiological response of the two cultivars. The tolerant cultivar differentially expressed a larger number of genes at 90 days, while in the sensitive cultivar the number of differentially expressed genes was higher in 30 days. Both cultivars perceived the lack of water, but the tolerant cultivar responded more slowly than the sensitive cultivar. The latter requires rapid activation of different water-deficit stress response mechanisms for its survival. This rapid activation of metabolic pathways in response to water stress does not appear to be the key mechanism of drought tolerance in sugarcane. There is still much to clarify on the molecular and physiological pattern of plants in response to drought.
650 $aDrought
650 $aGene expression
650 $aSaccharum
650 $aSugarcane
650 $aWater stress
650 $aCana de açúcar
650 $aDeficiência hídrica
653 $aExpressão genética
700 1 $aCARVALHO, F. M. S.
700 1 $aTELLES, B. R.
700 1 $aCASTRO, G. M. de
700 1 $aGIACHETTO, P. F.
700 1 $aVANTINI, J. S.
700 1 $aCARLIN, S. D.
700 1 $aCAZETTA, J. O.
700 1 $aPINHEIRO, D. G.
700 1 $aFERRO, M. I. T.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 16, n. 2, p. 1-20, 2017.
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